Biest Calculator for Estriol (E3) and Estradiol (E2) Ratio
Bi-est Compounding Calculator
Calculate Estriol (E3) and Estradiol (E2) amounts based on concentration and ratio
Calculation Results
Estriol (E3)
Estradiol (E2)
Total Summary
Medically Reviewed By
Adesh Pundir, RPh – Licensed Pharmacist since 2013
Verified for accuracy and clinical relevance.
Serving patients in Fort Worth, Texas with expertise in community pharmacy and medication safety.
Last reviewed: April 2026
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How our Bi-est compounding calculator works?
Our Bi-est compounding calculator simplifies the process of determining exact hormone amounts in your prescribed formulation. The tool requires three key inputs:
- First, enter your Bi-est concentration in mg/ml: this represents how much total estrogen is present in each milliliter of your preparation (e.g., 2 mg/ml means 2 milligrams of combined E3 and E2 per milliliter).
- Second, select your prescribed E3:E2 ratio from the available options ranging from 90:10 to 10:90. This determines what percentage of the total estrogen is estriol versus estradiol.
- Third, input the total amount you plan to use (in milliliters). This could represent your daily dose, weekly application, or any volume you need to calculate.
The calculator then performs three-step mathematics: it multiplies concentration by total amount to determine total estrogen content, calculates the E3 portion based on the first ratio number, and calculates the E2 portion based on the second ratio number.
Results display both in milligrams and grams including percentages of the total, providing clear understanding of exactly how much of each hormone component you’re receiving.
This tool serves educational purposes to help patients understand their hormone therapy and also it helps compounding pharmacist to calculate exact amount to be made. Understanding your specific Bi-est formulation empowers you to have more informed discussions with your provider about symptom management and therapy optimization.
Bi-Est Hormone Compounding Calculator:
Bi-est is a compounded bioidentical hormone therapy containing two forms of estrogen: estriol (E3) and estradiol (E2). Bi-est offers a balanced approach to hormone therapy by combining these naturally occurring hormones in specific ratios compounded for individual needs.
Estriol is considered the “weaker” estrogen with protective properties, while estradiol is the most potent form, responsible for many of estrogen’s primary effects. This combination aims to provide therapeutic benefits while potentially minimizing risks associated with stronger estrogens alone. Compounded Bi-est is typically available in various forms including creams, gels, capsules, and troches, allowing for personalized delivery methods based on patient preference and absorption needs.
Bi-est Compounding Benefits
The main benefit of Bi-est is that it can be custom-made just for you.
With regular hormone medicines, you get a standard formula that’s the same for everyone. But with Bi-est, a compounding pharmacist and your provider can work together to modify E3 and E2 amounts to match exactly what your body needs, based on your symptoms, health history, lab values. and how you feel. This personalized mix often works better for relieving symptoms and may cause fewer side effects.
Many doctors or providers like Bi-est for menopause treatment because:
- Estriol (E3) may help protect breast tissue
- Estradiol (E2) still helps with hot flashes, night sweats, vaginal dryness, and other menopause problems
Also, since Bi-est is made specially for you, it doesn’t have to contain:
- Artificial dyes
- Preservatives
- Common allergens
This makes it a good choice for people with sensitivities or allergies to ingredients in regular store-bought medicines.
Most common Bi-est formulations
Bi-est 50:50
Bi-Est 50:50 is a common hormone therapy that contains two forms of estrogen: estriol (50%) and estradiol (50%). This balanced estrogen ratio is often used in bioidentical hormone replacement therapy (BHRT) for women. Doctors may recommend Bi-Est 50:50 to help manage menopause symptoms like hot flashes, night sweats, mood swings, vaginal dryness, and low energy.
The 50:50 estrogen blend is popular because it offers a mix of gentle and strong estrogen support. Estriol together with estradiol is more powerful. Together, they help support hormone balance, bone health, and overall women’s wellness.
Many women choose Bi-Est because it is custom compounded and designed to closely match the body’s natural hormones.
Bi-est 80:20
Bi-Est 80:20 is a popular form of bioidentical hormone replacement therapy (BHRT) used for women. It contains 80% estriol and 20% estradiol. Many doctors prefer the 80:20 ratio because estriol is a gentler and weaker estrogen, making it safer for long-term use. Estradiol is stronger, so using a smaller amount helps reduce side effects.
The Bi-Est 80:20 ratio is often preferred over Bi-Est 50:50 because it provides estrogen support with lower estrogen strength. This makes it a good choice for women who are sensitive to hormones or want a more natural balance. It is commonly used to help with menopause symptoms such as hot flashes, night sweats, vaginal dryness, and mood changes.
Bi-Est 80:20 is available as cream, capsule, or troche and is custom compounded. Always talk to a healthcare provider to choose the right estrogen therapy for your needs.